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Specialty: Biochemistry
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Total 112299 results found since Jan 2013.
Molecular symmetry-constrained systematic search approach to structure solution of the coiled-coil SRGAP2 F-BARx domain
SRGAP2 (Slit – Robo GTPase-activating protein 2) is a cytoplasmic protein found to be involved in neuronal branching, restriction of neuronal migration and restriction of the length and density of dendritic postsynaptic spines. The extended F-BAR (F-BARx) domain of SRGAP2 generates membrane protrusions when expressed in COS-7 cells, while most F-BARs induce the opposite effect: membrane invaginations. As a first step to understand this discrepancy, the F-BARx domain of SRGAP2 was isolated and crystallized after co-expression with the carboxy domains of the protein. Diffraction data were collected from two significantly n...
Source: Acta Crystallographica Section D - November 28, 2016 Category: Biochemistry Authors: Sporny, M. Guez-Haddad, J. Waterman, D.G. Isupov, M.N. Opatowsky, Y. Tags: exhaustive search SRGAP2 F-BAR coiled coil molecular replacement research papers Source Type: research
HIF-1 α promotes the expression of syndecan-1 and inhibits the NLRP3 inflammasome pathway in vascular endothelial cells under hemorrhagic shock
Biochem Biophys Res Commun. 2022 Nov 1;637:83-92. doi: 10.1016/j.bbrc.2022.10.102. Online ahead of print.ABSTRACTHemorrhagic shock (HS) is a global life-threatening matter that causes massive mortality annually worldwide. Syndecan-1 (SDC1) is an important predictor and evaluation index for HS, but its mechanism involved in the HS development remain unclear. HS mice model and human umbilical vein endothelial cells (HUVECs) under hypoxia were applied to explore the relationship of SDC1 with HIF-1α and NLRP3 inflammasome in vascular ECs under HS. Transcriptome sequencing of isolated vascular ECs were conduct to search for hu...
Source: Biochemical and Biophysical Research communications - November 15, 2022 Category: Biochemistry Authors: Zhusheng Feng Yingnan Fan Jiangang Xie Shanshou Liu Chujun Duan Qianmei Wang Yuqin Ye Wen Yin Source Type: research
Baicalin, baicalein and wogonin inhibits high glucose-induced vascular inflammation in vitro and in vivo.
Abstract
Vascular inflammatory process has been suggested to play a key role in initiation and progression of atherosclerosis, a major complication of diabetes mellitus. Thus, in this study, we attempted to determine whether three structurally related polyphenols found in the Chinese herb Huang Qui, namely baicalin, baicalein, and wogonin, can suppress vascular inflammatory processes induced by high glucose (HG) in human umbilical vein endothelial cells (HUVECs) and mice. Data showed that HG induced markedly increased vascular permeability, monocyte adhesion, expressions of cell adhesion molecules (CAMs), formatio...
Source: BMB Reports - March 5, 2015 Category: Biochemistry Authors: Ku SK, Bae JS Tags: BMB Rep Source Type: research
Role of vascular smooth muscle cell in the inflammation of atherosclerosis.
Abstract
Atherosclerosis is a pathologic process occurring within the artery, in which many cell types, including T cell, macrophages, endothelial cells, and smooth muscle cells, interact, and cause chronic inflammation, in response to various inner- or outer-cellular stimuli. Atherosclerosis is characterized by a complex interaction of inflammation, lipid deposition, vascular smooth muscle cell proliferation, endothelial dysfunction, and extracellular matrix remodeling, which will result in the formation of an intimal plaque. Although the regulation and function of vascular smooth muscle cells are important in th...
Source: BMB Reports - January 6, 2014 Category: Biochemistry Authors: Lim S, Park S Tags: BMB Rep Source Type: research
Lignans from the stems and leaves of Brandisia hancei and their effects on VEGF-induced vascular permeability and migration of HRECs and DLAV formation in zebrafish.
Abstract
In our continuing search for novel antiangiogenic agents, a new lignan glycoside, (7R,8R)-1-(4-O-β-d-glucopyranosyl-3-methoxyphenyl)-2-{2-methoxy-4-[1-(E)-propene-3-ol]-phenoxyl}-propane-1,3-diol (1), along with three known lignans (2-4), were isolated from the 80% EtOH extract of Brandisia hancei stems and leaves. These isolates (1-4) were subjected to an in vitro bioassay to evaluate their effects on vascular endothelial growth factor (VEGF)-induced vascular permeability and migration of human retinal endothelial cells (HRECs). Of the compounds tested, compound 1 resulted in the greatest reduction in V...
Source: Bioscience, Biotechnology, and Biochemistry - December 15, 2014 Category: Biochemistry Authors: Lee IS, Kim YS, Jung SH, Yu SY, Kim JH, Sun H, Kim JS Tags: Biosci Biotechnol Biochem Source Type: research
Systematic review of clinical applications of monitoring muscle tissue oxygenation with near-infrared spectroscopy in vascular disease
Conclusion We found sufficient evidence to use NIRS in clinical setting for assessment of chronic compartment syndrome of lower extremities, and as surveillance tool for detection of free flap failure. So far, clinical relevance of routine use of NIRS in other vascular applications is less clear. Cut-off values to discriminate are not yet unanimous and better validation has to be awaited for.
Source: Microvascular Research - April 8, 2016 Category: Biochemistry Source Type: research
Roles of YAP in mediating endothelial cell junctional stability and vascular remodeling.
Abstract
Angiogenesis is complex processes involving dynamic interaction of various cell to cell interactions. Endothelial cell interactions, in particular, regulated by growth factors, inflammatory cytokines, or hemodynamic stress are critical in balancing vascular quiescence and activation. Yes-associated protein (YAP) as an effector of Hippo signaling is known to play significant roles in maintaining cellular homeostasis. However, its role in endothelial cells for angiogenic regulation remains relatively unexplored. Our recent study demonstrated the critical role of YAP in vascular endothelial cells and elucida...
Source: BMB Reports - July 14, 2015 Category: Biochemistry Authors: Choi HJ, Kwon YG Tags: BMB Rep Source Type: research
miR-15b induced by platelet-derived growth factor signaling is required for vascular smooth muscle cell proliferation.
Abstract
The platelet-derived growth factor (PDGF) signaling pathway is essential for inducing a dedifferentiated state of vascular smooth muscle cells (VSMCs). Activation of PDGF inhibits smooth muscle cell (SMC)-specific gene expression and increases the rate of proliferation and migration, leading to dedifferentiation of VSMCs. Recently, microRNAs have been shown to play a critical role in the modulation of the VSMC phenotype in response to extracellular signals. However, little is known about microRNAs regulated by PDGF in VSMCs. Herein, we identify microRNA-15b (miR-15b) as a mediator of VSMC phenotype regula...
Source: BMB Reports - October 25, 2013 Category: Biochemistry Authors: Kim S, Kang H Tags: BMB Rep Source Type: research
Structure and function of vascular endothelial growth factor and its receptor system.
Abstract
Vascular endothelial growth factor and its receptor (VEGF-VEGFR) system play a critical role in the regulation of angiogenesis and lymphangiogenesis in vertebrates. Each of the VEGF has specific receptors, which it activates by binding to the extracellular domain of the receptors, and, thus, regulates the angiogenic balance in the early embryonic and adult stages. However, de-regulation of the VEGF-VEGFR implicates directly in various diseases, particularly cancer. Moreover, tumor growth needs a dedicated blood supply to provide oxygen and other essential nutrients. Tumor metastasis requires blood vessels...
Source: BMB Reports - February 5, 2018 Category: Biochemistry Authors: Park SA, Jeong MS, Ha KT, Jang SB Tags: BMB Rep Source Type: research
In Vitro Selection of New DNA Aptamers for Human Vascular Endothelial Growth Factor 165.
Abstract
Two DNA aptamers that bind heparin-binding domain (HBD) of the human vascular endothelial growth factor 165 (VEGF-165) have been previously reported. Although VEGF-165 is a homodimeric protein and the two aptamers have different sequences and secondary structures, these aptamers appear to occupy the same binding site and cannot form the 2:1 aptamer/protein complex, making them unsuitable for creating a higher affinity dimeric DNA aptamer. This has motivated us to conduct a new in vitro selection experiment to search for new VEGF-165 binding DNA aptamers with different properties. We undertook a multi-stre...
Source: Chembiochem - March 15, 2020 Category: Biochemistry Authors: Manochehry S, Gu J, McConnell EM, Salena B, Li Y Tags: Chembiochem Source Type: research
Comparative database search engine analysis on massive tandem mass spectra of pork-based food products for halal proteomics
This study highlights the use of different database search engines (Comet, X! Tandem, ProteinProspector, and ProteinPilot™) and their respective algorithms to analyse 135 raw tandem mass spectrometry data files from 15 types of pork-based food products. This is the first attempt that has compared different database search engines in the context of halal proteomics to ensure the effectiveness of controlling the FDR. Previous studies were just focused on the advantages of a certain algorithm over another. Moreover, other previous studies also have mainly reported the use of mass spectrometry-based shotgun proteomics for me...
Source: Journal of Proteomics - April 24, 2021 Category: Biochemistry Authors: Siti Hajar Amir Mohd Hafis Yuswan Wan Mohd Aizat Muhammad Kamaruzaman Mansor Mohd Nasir Mohd Desa Yus Aniza Yusof Lai Kok Song Shuhaimi Mustafa Source Type: research
Fibroblast growth factor 21 inhibits vascular calcification by ameliorating oxidative stress of vascular smooth muscle cells
In conclusion, FGF21 could inhibit vascular calcification, partly by restoring the level of antioxidant SOD and reducing vascular oxidative stress. This study provides further evidence for FGF21 as a candidate drug for cardiovascular protective agents.PMID:36773338 | DOI:10.1016/j.bbrc.2023.01.054
Source: Biochemical and Biophysical Research communications - February 11, 2023 Category: Biochemistry Authors: Yingkai Li Songyuan He Cong Wang Wen Jian Xueqian Shen Yuchen Shi Jinghua Liu Source Type: research
AA_stat: Intelligent profiling of in vivo and in vitro modifications from open search results
J Proteomics. 2021 Aug 10:104350. doi: 10.1016/j.jprot.2021.104350. Online ahead of print.ABSTRACTCharacterization of post-translational modifications is among the most challenging tasks in tandem mass spectrometry-based proteomics which has yet to find an efficient solution. The ultra-tolerant (open) database search attempts to meet this challenge. However, interpretation of the mass shifts observed in open search still requires an effective and automated solution. We have previously introduced the AA_stat tool for analysis of amino acid frequencies at different mass shifts and generation of hypotheses on unaccounted in v...
Source: Journal of Proteomics - August 14, 2021 Category: Biochemistry Authors: Lev I Levitsky Julia A Bubis Mikhail V Gorshkov Irina A Tarasova Source Type: research
